The Penguins lost 4-2 to Buffalo Tuesday night at Consol Energy Center when Steve Ott batted the game winner past Marc-Andre Fleury from mid-air late in the third period.
The shot came off a rebound that squirted above Fluery, and was reviewed by officials, who determined there was no high-sticking penalty.
The goal, at 17:26, served as the winner, and was followed up with an empty netter by Thomas Vanek.
Pittsburgh's Jerome Iginla had tied the game 2-2 with a power play goal from the left circle, that was screened by Brendan Morrow at 11:02 of the third period.
Buffalo's Jochen Hecht had wristed one by Fleury at 6:11 to give the Sabres a 2-1 lead.
The Penguins got some star power back with the return of Evgeni Malkin to the lineup on Tuesday night but lost defenseman Brooks Orpik to a lower body injury when he never returned to the ice after the first period.
The game had little implications on the standings of either team, and was rescheduled from Saturday when the Penguins game at Boston was pushed back a day.
The team returned in the wee hours on Tuesday from a 3-1 win at Ottawa to turn around and face Buffalo.
Buffalo took the game's first lead when Thomas Vanek one-timed a backhanded feed from behind the net past Marc-Andre Fleury at 12:49 to make it 1-0 Sabres.
The Penguins responded just 20 seconds later when Brandon Sutter dished the puck to Chris Kunitz on the left wing as the pair entered the Sabres zone off the rush.
Kunitz fired a wrister over Ryan Miller's glove to make it 1-1 at 13:09 of the first.
In the second period Fleury gloved a Thomas Vanek breakaway just as 4-on-4 play expired.
Vanek had been whistled for goaltender interference and Jussi Jokinen for holding at 6:25.
Pittsburgh went on back-to-back power plays at 17:44 and 19:49 of the period and ended the scoreless period with flurry of shots.
The Penguins opened the third period with 1:50 left on a power play from an Adam Pardy cross-check of Morrow in the crease, but failed to convert.